random primer plus extension labeling system Search Results


random primer plus extension labeling system  (NEN Life Science)
90
NEN Life Science random primer plus extension labeling system
Random Primer Plus Extension Labeling System, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primer plus extension labeling system/product/NEN Life Science
Average 90 stars, based on 1 article reviews
random primer plus extension labeling system - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
random primer extension kit  (NEN Life Science)
90
NEN Life Science random primer extension kit
Random Primer Extension Kit, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primer extension kit/product/NEN Life Science
Average 90 stars, based on 1 article reviews
random primer extension kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
radioisotope-labeled by random primer extension  (Boehringer Mannheim)
90
Boehringer Mannheim radioisotope-labeled by random primer extension
Radioisotope Labeled By Random Primer Extension, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/radioisotope-labeled by random primer extension/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
radioisotope-labeled by random primer extension - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
labeled by primer extension from random hexanucleotides  (Boehringer Mannheim)
90
Boehringer Mannheim labeled by primer extension from random hexanucleotides
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Labeled By Primer Extension From Random Hexanucleotides, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/labeled by primer extension from random hexanucleotides/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
labeled by primer extension from random hexanucleotides - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
random primer extension kit rediprime dna labeling system  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc random primer extension kit rediprime dna labeling system
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Random Primer Extension Kit Rediprime Dna Labeling System, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primer extension kit rediprime dna labeling system/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
random primer extension kit rediprime dna labeling system - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
nick-translation kit  (NEN Life Science)
90
NEN Life Science nick-translation kit
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Nick Translation Kit, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nick-translation kit/product/NEN Life Science
Average 90 stars, based on 1 article reviews
nick-translation kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
32p labelling of dna by random primer extension megaprime kit  (Amersham Pharmacia Biotech Ltd)
90
Amersham Pharmacia Biotech Ltd 32p labelling of dna by random primer extension megaprime kit
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
32p Labelling Of Dna By Random Primer Extension Megaprime Kit, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/32p labelling of dna by random primer extension megaprime kit/product/Amersham Pharmacia Biotech Ltd
Average 90 stars, based on 1 article reviews
32p labelling of dna by random primer extension megaprime kit - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
pcr fragment primers 77±1 77±2 labeled 32p random primer extension  (Lofstrand)
90
Lofstrand pcr fragment primers 77±1 77±2 labeled 32p random primer extension
Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random <t>hexanucleotides</t> (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.
Pcr Fragment Primers 77±1 77±2 Labeled 32p Random Primer Extension, supplied by Lofstrand, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr fragment primers 77±1 77±2 labeled 32p random primer extension/product/Lofstrand
Average 90 stars, based on 1 article reviews
pcr fragment primers 77±1 77±2 labeled 32p random primer extension - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.

Journal:

Article Title: Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

doi: 10.1093/emboj/19.11.2629

Figure Lengend Snippet: Fig. 5. Gal4-VP16 directs the HAT activity of the SAGA complex to promoter-proximal nucleosomes. The experimental set-up for these ‘scanning ChIPS’ is similar to that used in Figure ​Figure4:4: the template was incubated in the absence (–) or presence (+) of Gal4-VP16, and competitor chromatin was added where indicated. Next, the reactions were incubated with SAGA and acetyl-CoA (or mock acetylated in the absence of HAT complex), MNase digested and immunoprecipitated with anti-acetylated H3 antibody. DNA was extracted from the bound and unbound fractions and slot-blotted. The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). (A) Diagram showing the localization of the different probes when the plasmid is digested with BglI. (B) Average values and standard deviation of normalized data from three repeats of the experiment. The background signal (–HAT) was subtracted from the values obtained in the presence of SAGA for each condition. The numbers under the graph show the ratio of proximal (average of +A and –A) versus distal (average of +C and –C) signal for each condition tested. ND, not determined. (C) The reconstituted array was pre-incubated with Gal4-VP16 and competitor chromatin was added. Then, the template was acetylated by SAGA in the presence of acetyl-CoA, reactions were digested with MNase (+) or mock-digested (–), and the immunoprecipitation and slot blot were carried out as described above.

Article Snippet: The membranes were hybridized successively with a series of short probes (between 250 and 300 bp) that scan the length of the template, generated by PCR and labeled by primer extension from random hexanucleotides (Boehringer Mannheim). ( A ) Diagram showing the localization of the different probes when the plasmid is digested with Bgl I. ( B ) Average values and standard deviation of normalized data from three repeats of the experiment.

Techniques: Activity Assay, Incubation, Immunoprecipitation, Generated, Labeling, Plasmid Preparation, Standard Deviation, Dot Blot